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Assessment and Characterization of Hyaloid Vessels in Mice
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Médecine
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Journal JoVE Médecine
Assessment and Characterization of Hyaloid Vessels in Mice

Assessment and Characterization of Hyaloid Vessels in Mice

DOI:
10.3791/59222-v

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08:22 min

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May 15, 2019

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, , ,
1Department of Ophthalmology, Boston Children’s Hospital,Harvard Medical School, 2The Manton Center for Orphan Disease Research, Boston Children’s Hospital,Harvard Medical School

Chapitres

  • 00:04Titre
  • 01:31In Vivo Hyaloid Vessel Imaging
  • 02:23Hyaloid Vessel Harvest for Ex Vivo Visualization
  • 04:50Hyaloid Vessel Flat Mounting and Ex Vivo Imaging
  • 05:57Results: Representative In Vivo and Ex Vivo Visualization
  • 07:24Conclusion

Summary

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This protocol describes both in vivo and ex vivo methods to fully visualize and characterize hyaloid vessels, a model of vascular regression in mouse eyes, using optical coherence tomography and fundus fluorescein angiography for the live imaging and ex vivo isolation and subsequent flat mount of hyaloid for quantitative analysis.

Tags

Hyaloid VesselsVascular RegressionPersistent Fetal VasculatureAngiogenesisOptical Coherence TomographyFundus Fluorescein AngiographyMicrosurgery ForcepsVitreous BodyEyeball Dissection

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