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A Galleria mellonella Oral Administration Model to Study Commensal-Induced Innate Immune Responses
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Immunologie et infection
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Journal JoVE Immunologie et infection
A Galleria mellonella Oral Administration Model to Study Commensal-Induced Innate Immune Responses

A Galleria mellonella Oral Administration Model to Study Commensal-Induced Innate Immune Responses

DOI:
10.3791/59270-v

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06:32 min

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March 21, 2019

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, ,
1Department for Medical Microbiology, Hygiene, Interfacultary Institute for Microbiology, Infection Medicine,University of Tübingen

Chapitres

  • 00:04Titre
  • 00:28G. mellonella Rearing and Larva Preparation and Force Feeding
  • 01:53Orally-Administered Larva Processing and RNA Isolation
  • 04:22Results: Representative Commensal-Induced Gene and Protein Expression in G. mellonella
  • 05:33Conclusion

Summary

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Traduction automatique

Here, we provide a detailed protocol for an oral administration model using Galleria mellonella larvae and how to characterize induced innate immune responses. Using this protocol, researchers without practical experience will be able to use the G. mellonella force-feeding method.

Tags

Galleria MellonellaOral AdministrationInnate Immune ResponsesCommensal BacteriaInfection ModelPathogenic BacteriaLarvaeSubstrateForce FeedingHomogenizationRNA ExtractionTRIzol1-Bromo-3-chloropropane

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