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Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit
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Journal JoVE Biologie
Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit

Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit

DOI:
10.3791/59290-v

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08:43 min

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April 19, 2019

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, , , , , , , , , , ,
1Division of Gastroenterology,Perelman School of Medicine, University of Pennsylvania

Chapitres

  • 00:04Titre
  • 00:54Tissue Preparation
  • 03:10Lipid Droplet (LD) Isolation
  • 05:18LD Washing
  • 06:38Results: Analysis of LDs after Isolation
  • 08:19Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol establishes a novel method of lipid droplet isolation and purification from mouse livers, using a well-established endoplasmic reticulum isolation kit.

Tags

Lipid DropletOrganelle IsolationLiverMouseERLysosomeHomogenizationCentrifugationIE BufferRapid Isolation

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