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A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents
Journal JoVE
Médecine
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Journal JoVE Médecine
A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents

A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents

DOI:
10.3791/59295-v

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04:19 min

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April 19, 2019

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, , , , ,
1Bayer School of Natural and Environmental Sciences,Duquesne University, 2Department of Biological Sciences,Duquesne University, 3Chronic Pain Research Consortium,Duquesne University, 4Graduate School of Pharmaceutical Sciences,Duquesne University

Chapitres

  • 00:04Titre
  • 00:47Near-infrared (NIRF)-containing Agent Tail Vein Injection
  • 03:02Results: Representative NIRF-based Nanoemulsion Tail Vein Imaging
  • 03:40Conclusion

Summary

Traduction automatique

Traduction automatique

Here we present a method to validate tail vein injections in rats by utilizing near-infrared fluorescence imaging data from dyes incorporated into agents or biological probes. The tail is imaged before and after the injection, the fluorescent signal is quantified, and an assessment of the injection quality is made.

Tags

Tail Vein InjectionNear-infrared-labeled AgentsFluorescence ImagingAnesthetized AnimalTail VasculatureLateral Tail Vein25-27 Gauge NeedleNeedle PlacementSyringe InjectionCatheterBlood FlashbackQuality ControlAnimal StudiesReproducibility

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