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Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
 

Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales

Article DOI: 10.3791/59356-v • 09:56 min • August 21st, 2019
August 21st, 2019 •
1, 2, 3, 4, 1, 1
1Stream Biofilm and Ecosystem Research Laboratory, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne, 2Plateforme technique, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne, 3Water Chemistry and Water Technology, Engler-Bunte-Institut, Karlsruhe Institute of Technology, 4Thorlabs GmbH

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Microbial biofilms form complex architectures at interphases and develop into highly scale-dependent spatial patterns. Here, we introduce an experimental system (hard- and software) for the automated acquisition of 3D optical coherence tomography (OCT) datasets. This toolset allows the non-invasive and multi-scale characterization of biofilm morphogenesis in space and time.

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Keywords: 3D Optical Coherence Tomography Biofilm Morphogenesis Automated System Large Spatial Scales Extended Time Periods Ecosystem Processes Natural Systems Engineered Environments Precision Positioning Device Plexiglass Flume GRBL Server Microfluidic Devices Flow Chambers Flumes Filtration Systems OCT System Image Acquisition Positioning Device Dovetail Holder Immersion Adapter
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