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Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
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Journal JoVE Génétique
Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

DOI:
10.3791/59394-v

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09:31 min

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April 19, 2019

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, , , , , , ,
1Department of Developmental Physiology,Centre for Organismal Studies (COS) Heidelberg, 2Department of Cell Biology,Centre for Organismal Studies (COS) Heidelberg, 3Department of Stem Cell Biology,Centre for Organismal Studies (COS) Heidelberg

Chapitres

  • 00:04Titre
  • 00:53Induction of Trans-activation in Arabidopsis Driver Lines: Root
  • 01:49Induction of Trans-activation in Arabidopsis Driver Lines: Stem
  • 02:54Induction of Trans-activation in Arabidopsis Driver Lines: Shoot Apical Meristem (SAM)
  • 03:35Imaging of Reporter Expression in Arabidopsis Driver Lines: Root
  • 04:28Imaging of Reporter Expression in Arabidopsis Driver Lines: Stem
  • 06:02Imaging of Reporter Expression in Arabidopsis Driver Lines: Shoot Apical Meristem
  • 07:41Results: Analysis of Cell Type-Specific Expression in Arabidopsis thaliana
  • 08:38Conclusion

Summary

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Traduction automatique

Here, we describe the generation and application of a set of transgenic Arabidopsis thaliana lines enabling inducible, tissue-specific expression in the three main meristems, the shoot apical meristem, the root apical meristem, and the cambium.

Tags

Arabidopsis ThalianaInducible ExpressionCell Type-specificLhGR-mediated Trans-activationGreen Gate CloningMeristemsConfocal MicroscopyDEX InductionSilwet L-77Shoot Apical Meristem

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