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Immunology and Infection

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JoVE Journal Immunology and Infection
An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes
 

An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes

Article DOI: 10.3791/59470-v • 10:17 min • April 26th, 2019
April 26th, 2019 •
1,2,3,4, 3,6, 3,5, 3,5, *1,2,4, *2,3,5
1Department of Endocrinology & Metabolism Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, 2Berlin Institute of Health (BIH), 3Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, 4DZHK (German Centre for Cardiovascular Research), 5Institute of Medical Immunology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, 6Julius Wolff Institute (JWI) and Center for Musculoskeletal Surgery, Charité - Universitätsmedizin Berlin, Berlin, Germany, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin
* These authors contributed equally

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résumé
Automatic Translation

A simple and reliable diet-induced rodent animal model for nonalcoholic steatohepatitis (NASH) is described, achieved through non-SPF housing of the animals and administration of a specific high-fat diet. We describe identification of hepatic and adipose immune cell subsets to recapitulate human immunological conditions by exposing mice to environmental germs.

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Keywords: Murine Model Nonalcoholic Steatohepatitis Type 2 Diabetes Immunometabolism Adipose Tissue Hepatic Tissue Immune Cells Dissociation Flow Cytometry Adipose Tissue Digest Stromal Vascular Fraction ACK Lysis Liver
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