01:33Lentivirus-Renilla-Luciferin-Red Fluorescent Protein (LV-Rluc-RFP; RR) and LV-Rluc-RFP-Herpes Simplex Virus Truncated Thymidine Kinase (HSV-ttk) (LV-Rluc-RFP-TTK; RT) Packaging and Production
03:38LV-RR and LV-RRT Lentiviral Transduction for Gene Expression in 4T1 Cells
04:37Drug Screening and LV-RR and LV-RRT-Transduced 4T1 Cell Identification
05:18Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)-Firefly Luciferase (Fluc)-Knockin (KI) Mice and Tumor-Bearing Mouse Model
06:50Dual Bioluminescence Tumor (Rluc) and Angiogenesis (Fluc) Imaging
07:58Results: Representative In Situ Tumor Growth and Angiogenesis Visualization and Analysis
Ce protocole décrit l'établissement d'un modèle de souris tumeur-portant pour surveiller la progression de tumeur et l'angiogenèse en temps réel par la formation image double de bioluminescence.