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Dual bioluminesens Imaging av tumor progresjon og angiogenese
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Médecine
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Journal JoVE Médecine
Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis

Dual bioluminesens Imaging av tumor progresjon og angiogenese

DOI:
10.3791/59763-v

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10:56 min

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August 01, 2019

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, , , ,
1Nankai University School of Medicine, 2State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy,Nankai University

Chapitres

  • 00:04Titre
  • 01:33Lentivirus-Renilla-Luciferin-Red Fluorescent Protein (LV-Rluc-RFP; RR) and LV-Rluc-RFP-Herpes Simplex Virus Truncated Thymidine Kinase (HSV-ttk) (LV-Rluc-RFP-TTK; RT) Packaging and Production
  • 03:38LV-RR and LV-RRT Lentiviral Transduction for Gene Expression in 4T1 Cells
  • 04:37Drug Screening and LV-RR and LV-RRT-Transduced 4T1 Cell Identification
  • 05:18Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)-Firefly Luciferase (Fluc)-Knockin (KI) Mice and Tumor-Bearing Mouse Model
  • 06:50Dual Bioluminescence Tumor (Rluc) and Angiogenesis (Fluc) Imaging
  • 07:58Results: Representative In Situ Tumor Growth and Angiogenesis Visualization and Analysis
  • 09:52Conclusion

Summary

Traduction automatique

Traduction automatique

Denne protokollen beskriver etablering av en tumor-bærende mus modell for å overvåke tumorprogresjon og angiogenese i sanntid av dual bioluminesens Imaging.

Tags

Keywords: Bioluminescence ImagingTumor ProgressionAngiogenesisBreast CancerFirefly LuciferaseRenilla LuciferaseLentivirus293T CellsTumor MetastasisAntitumor Drug ScreeningOncology ResearchTherapeutic StrategiesTumor RegressionMolecular Processes

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