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番茄根转化后与拉子托尼亚索拉纳卡鲁姆接种，直接遗传分析细菌性威尔特病
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Journal JoVE Génétique
Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
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番茄根转化后与拉子托尼亚索拉纳卡鲁姆接种，直接遗传分析细菌性威尔特病

DOI:
10.3791/60302-v

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09:05 min

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March 11, 2020

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, , , ,
1Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes of Biological Sciences,Chinese Academy of Sciences, 2University of Chinese Academy of Sciences, 3Department of Soil Microbiology and Symbiotic Systems,Estación Experimental del Zaidín (CSIC)

Chapitres

  • 00:04Introduction
  • 01:03Preparation of Tomato Plants and Agrobacterium Rhizogenes
  • 01:55Plant Transformation and Selection
  • 04:01Transfer to Inoculation Pots
  • 04:40Soil-Drenching Inoculation
  • 05:41Results: Genetic Analysis of Bacterial Wilt Disease
  • 08:20Conclusion

Summary

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Traduction automatique

在这里，我们提出了一种用于番茄根转化的通用方法，然后用Ralstonia索兰卡鲁姆接种，对细菌枯萎病的研究进行直接的基因分析。

Tags

TomatoRoot TransformationRalstonia SolanacearumBacterial WiltGenetic AnalysisAgrobacterium RhizogenesIn VitroSterilitySeedling Manipulation

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