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Biochemistry

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JoVE Journal Biochemistry
An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
 

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Article DOI: 10.3791/60449-v • 07:37 min • January 9th, 2020
January 9th, 2020 •
1,2, 1,2, 1
1Biodynamics Research Center (BDR), RIKEN, 2Research Center, Misr International University

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This protocol presents an integrated Raman spectroscopy-mass spectrometry (MS) platform that is capable of achieving single-cell resolution. Raman spectroscopy can be used to study cellular response to drugs, while MS can be used for targeted and quantitative analysis of drug uptake and metabolism.

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Keywords: Raman Spectroscopy Mass Spectrometry Single-cell Analysis Drug Uptake Metabolism Cellular Heterogeneity Cancer Resistance Metabolomics Spectral Imaging Micromanipulator Cell Culture Tamoxifen DMSO Spectrophotometer Calibration Micro-chamber
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