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Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
 

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Article DOI: 10.3791/60451-v • 08:25 min • October 31st, 2019
October 31st, 2019 •
*1,2,3, *1,2, *1,2, 2,3, 1,2
1Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, 2Centre for Synthetic and Systems Biology, University of Edinburgh, 3Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences, University of Edinburgh
* These authors contributed equally

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résumé
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Here, we present a protocol describing how to i) assemble a self-replicating vector using the CyanoGate modular cloning toolkit, ii) introduce the vector into a cyanobacterial host by conjugation, and iii) characterize transgenic cyanobacteria strains using a plate reader or flow cytometry.

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Keywords: Genetic Modification Cyanobacteria Conjugation CyanoGate Modular Cloning Synthetic Biology Renewable Biotechnology Triparental Mating Transformation Synechocystis Synechococcus E. Coli Helper Strain Plasmids
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