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Developmental Biology

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JoVE Journal Developmental Biology
Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids
 

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Article DOI: 10.3791/60470-v • 08:15 min • November 15th, 2019
November 15th, 2019 •
1, 1, 1, 2, 1
1Department of Gastroenterology and Hepatology, Tytgat Institute for Intestinal and Liver Research, Amsterdam UMC, AG&M, University of Amsterdam, 2Department of Pediatrics, Amsterdam UMC, University of Amsterdam

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This protocol describes how to mimic suckling-to-weaning transition in vitro using mouse late fetal intestinal organoids cultured for 30 days.

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Suckling-to-weaning Transition In Vitro Fetal Intestinal Organoids Mouse Fetal Intestinal Cells Maturation Molecular Mechanisms Modulators Experimental Animals Gut Maturation Primary Intestinal Cells Embryonic Day 18-20 Forceps Small Intestine Colon Razor Blade PBS EDTA
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