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Immunologie et infection
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Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain
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Immunologie et infection
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Journal JoVE Immunologie et infection
Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

DOI:
10.3791/60510-v

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10:40 min

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October 27, 2019

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, , , , ,
1Axe Neurosciences,Centre de Recherche du CHU de Québec-Université Laval, 2Département de Médecine Moléculaire, Faculté de Médecine,Université Laval, 3Integrated Program in Neuroscience,McGill University, 4Center for Brain Immunology and Glia (BIG),University of Virginia

Chapitres

  • 00:04Titre
  • 01:00Density and Distribution Analysis
  • 05:50Morphology Analysis
  • 09:01Results: Immunofluorescence Staining Using IBA1 and TMEM119
  • 10:06Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol describes a step-by-step workflow for immunofluorescent costaining of IBA1 and TMEM119, in addition to analysis of microglial density, distribution, and morphology, as well as peripheral myeloid cell infiltration in mouse brain tissue.

Tags

ImmunofluorescenceIBA1TMEM119MicrogliaPeripheral Myeloid CellDensityMorphologyInfiltrationMouse BrainFijiImageJNearest Neighbor DistanceScaleComposite ImageChannel ToolFreehand SelectionSelection BrushRegion Of Interest

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