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A High Output Method to Isolate Cerebral Pericytes from Mouse
Journal JoVE
Biologie
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Journal JoVE Biologie
A High Output Method to Isolate Cerebral Pericytes from Mouse

A High Output Method to Isolate Cerebral Pericytes from Mouse

DOI:
10.3791/60588-v

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06:49 min

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January 14, 2020

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, , , , ,
1Laboratory of the Blood Brain Barrier,University of Artois, 2Department of Neurology, Universitätsmedizin Mannheim,Heidelberg University

Chapitres

  • 00:04Titre
  • 00:44Brain Tissue Recovery and Meninges Removal
  • 01:31Homogenization
  • 02:18Vascular Fraction Isolation
  • 04:32Cerebral Pericyte Proliferation
  • 05:25Results: Representative Cell Morphology, Marker Expression, and Culture Purity
  • 06:25Conclusion

Summary

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Traduction automatique

We present a protocol for the extraction of murine cerebral pericytes. Based on an antibiotic-free enrichment oriented pericyte extraction, this protocol is a valuable tool for in vitro studies providing high purity and high yield, thus decreasing the number of experimental animals used.

Tags

Cerebral PericytesPericyte IsolationMouse BrainIn Vitro StudiesCell CultureCell YieldHigh PurityHomogenizationVascular FractionBovine Serum Albumin DextranCell SuspensionFiltration

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