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Immunologie et infection
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Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

DOI:
10.3791/60681-v

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12:27 min

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January 03, 2020

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, , , , , , ,
1Department of Microbiology and Immunology,Montana State University, 2University Communications,Montana State University

Chapitres

  • 00:00Titre
  • 00:44Polymorphonuclear Leukocyte Purification
  • 04:34Cytotoxicity Assay of S. aureus Extracellular Proteins Against Human Polymorphonuclear Leukocytes
  • 06:16S. aureus Cytotoxicity Against Human Polymorhonuclear Leukocytes Following Phagocytosis
  • 08:40Résultats
  • 11:02Conclusion

Summary

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Traduction automatique

This protocol describes a method for the purification of polymorphonuclear leukocytes from whole human blood and two distinct assays that quantify the cytotoxicity of Staphylococcus aureus against these important innate immune cells.

Tags

Staphylococcus AureusCytotoxicityPolymorphonuclear LeukocytesPMNsNeutrophilsBi-component LeukotoxinsMembrane IntegrityInnate Immune CellsCentrifugationDensity GradientsHeparinized Whole BloodDextranFicollCell IsolationCell Separation

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