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使用基因组-规模CRISPR/Cas9基因屏幕进行细胞表面受体识别
Journal JoVE
Génétique
This content is Free Access.
Journal JoVE Génétique
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
DOI:

08:49 min

June 06, 2020

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Chapitres

  • 00:04Introduction
  • 01:07Quantification and Oligomerization of Monomeric Biotinylated Protein
  • 03:26Genetic Screening for Cell Surface Binding
  • 05:13Bioinformatics Analysis to Identify the Receptor and Related Pathways
  • 06:05Results: Genetic Screens for the Identification of Cell Surface Binding Partners
  • 07:57Conclusion

Summary

Traduction automatique

本手稿描述了一种基于基因组规模的细胞筛选方法,用于识别细胞外受体-配体相互作用。

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