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Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts
 

Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts

Article DOI: 10.3791/60909-v • 10:45 min • March 12th, 2020
March 12th, 2020 •
1, 1, 1,2, 1
1Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, 2American Red Cross, National Headquarters

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résumé
Automatic Translation

Obtaining a pure population of fibroblasts is crucial to studying their role in wound repair and fibrosis. Described here is a detailed method to isolate fibroblasts and myofibroblasts from uninjured and injured mouse hearts followed by characterization of their purity and functionality by immunofluorescence, RTPCR, fluorescence-assisted cell sorting, and collagen gel contraction.

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Keywords: Cardiac Fibroblasts Myofibroblasts Isolation Characterization Flow Cytometry Bead-based Isolation Tissue Dissection Collagenase Digestion Cell Culture
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