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A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

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Biologie du développement

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Journal JoVE Biologie du développement

A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

DOI:

10.3791/60955-v

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07:41 min

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August 06, 2020

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Amir Bagheri², Seyedeh Fatemeh Razavipour, Claes Wahlestedt, Seyed Javad Mowla, Mohammad Ali Faghihi⁴

¹Department of Molecular Genetics, Faculty of Biological Sciences,Tarbiat Modares University, ²Center for Therapeutic Innovation and Department of Psychiatry & Behavioral Sciences,University of Miami Miller School of Medicine, ³Department of Biochemistry and Molecular Biology,University of Miami Miller School of Medicine, ⁴Persian BayanGene Research and Training Center

Chapitres

00:04Introduction
00:51Passaging and Freezing the hNPCs
02:50Image Acquisition and Analysis
04:25Neurotoxicity Assessment
06:10Results: Neurite Outgrowth Assay and Neurotoxicity Assessment with Small Molecule Compounds
07:13Conclusion

Summary

Traduction automatique

The presented protocol describes a method for a neurite outgrowth assay and neurotoxicity assessment of small molecule compounds.

Tags

Neurite Outgrowth Assay Neurotoxicity Assessment Human Neural Progenitor Cells Neurons Induced Pluripotent Stem Cells Embryonic Stem Cells Neurosphere Cell Dissociation Cell Culture Cryopreservation Image Analysis Fiji ROI Manager Freehand Line

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