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Immunology and Infection

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JoVE Journal Immunology and Infection
Quantifying Human Norovirus Virus-like Particles Binding to Commensal Bacteria Using Flow Cytometry
 

Quantifying Human Norovirus Virus-like Particles Binding to Commensal Bacteria Using Flow Cytometry

Article DOI: 10.3791/61048-v • 07:02 min • April 29th, 2020
April 29th, 2020 •
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1Microbiology and Cell Science Department, University of Florida

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The goal of this protocol is to quantify binding of the eukaryotic pathogen human norovirus to bacteria. After performing an initial virus-bacterium attachment assay, flow cytometry is used to detect virally-bound bacteria within the population.

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Keywords: Virus-bacterial Interactions Human Norovirus Virus-like Particles Flow Cytometry Enterobacter Cloacae Bacterial Quantification Serial Dilutions Optical Density Colony Forming Units
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