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Immunology and Infection

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JoVE Journal Immunology and Infection
Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis
 

Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis

Article DOI: 10.3791/61180-v • 09:16 min • June 18th, 2020
June 18th, 2020 •
1, 2, 1, 1,3
1Department of Medicine, Monash University, 2Monash Micro Imaging, Monash University, 3Immunology Department, Monash Health

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résumé
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Extracellular DNA (ecDNA) released during cell death is proinflammatory and contributes to inflammation. Measurement of ecDNA at the site of injury can determine the efficacy of therapeutic treatment in the target organ. This protocol describes the use of a machine learning tool to automate measurement of ecDNA in kidney tissue.

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Keywords: Supervised Machine Learning Semi-quantification Extracellular DNA Glomerulonephritis Kidney Histology Antigen Retrieval Immunofluorescence Staining Sudan Black Quenching
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