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脑毛细管中培养细胞细胞钙测量和钙成像研究

Journal JoVE
Neurosciences

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Journal JoVE Neurosciences

Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies

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脑毛细管中培养细胞细胞钙测量和钙成像研究

DOI:

10.3791/61253-v

•

09:33 min

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May 27, 2020

•

Sofie Hørlyck, Hans Christian Cederberg Helms, Birger Brodin

¹Department of Pharmacy, The Faculty of Health and Medical Sciences,University of Copenhagen

Chapitres

00:04Introduction
00:41Isolation of Primary Pericytes from Bovine Brain Capillaries
03:01Loading of Pericytes with Calcium Indicator Dye in a Plate Reader Setup
05:18Live Imaging of Intracellular Ca²⁺ Levels
07:11Results: Pericyte Isolation and Calcium Measurements
09:02Conclusion

Summary

Traduction automatique

脑毛细管性信号是调节血脑屏障特性和血流的基本参与者。该协议描述了如何分离、培养脑毛细管腹膜，在细胞类型方面进行特征分析，并应用于用荧光探针研究细胞内钙信号。

Tags

Keywords: Brain Capillary Pericytes Primary Cell Culture Calcium Imaging Intracellular Signaling Cell Isolation Cell Detachment DMEM Trypsin-EDTA Centrifugation Cell Confluency Microscopy

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