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On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis
Journal JoVE
Médecine
This content is Free Access.
Journal JoVE Médecine
On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis

On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis

DOI:
10.3791/61260-v

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06:48 min

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May 31, 2020

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, , , , , , , , ,
1Department of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz,Nicolaus Copernicus University in Torun, 2Department of Neurosurgery,10th Military Research Hospital and Polyclinic, 3Division of Preventive Medicine and Healthy Policy, Collegium Medicum in Bydgoszcz,Nicolaus Copernicus University in Torun

Chapitres

  • 00:04Introduction
  • 00:34Solid Phase Microextraction (SPME) Device Preparation and Sample Collection
  • 02:45Metabolomic Analysis Sample Preparation
  • 03:56Lipidomic Analysis Sample Preparation
  • 05:07Results: Representative Lipidomic and Metabolomic Tumor Analyses
  • 06:05Conclusion

Summary

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The manuscript presents on-site sampling of human brain tumors with solid phase microextraction followed by their biochemical profiling towards the discovery of biomarkers.

Tags

On-site SamplingBrain TumorMetabolomicsLipidomicsSolid Phase MicroextractionRapid Intraoperative DiagnosticsLCMSSample PreparationDesorptionTumor Extraction

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