/
/
Dissection, Immunohistochemistry and Mounting of Larval and Adult Drosophila Brains for Optic Lobe Visualization
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:00Introduction
- 00:35Larval Brain Dissection
- 02:23Adult Brain Dissection
- 04:03Mounting Larval Brains
- 06:06Mounting Adult Brains
- 08:02Representative Images of Larval and Adult Optic Lobes
- 10:47Conclusion
This protocol describes three steps to prepare larval and adult Drosophila optic lobes for imaging: 1) brain dissections, 2) immunohistochemistry and 3) mounting. Emphasis is placed on step 3, as distinct mounting orientations are required to visualize specific optic lobe structures.
