/
/
Akış SitometriSi-Bazlı Bir Test ile Plasmodium falciparum Parazitlerine Doğal Olarak Edinilmiş Fagositoz-Indükleyen Antikorların Ölçülmesi
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences,University of Copenhagen, 2West African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology,University of Ghana, 3Department of Immunology,Noguchi Memorial Institute for Medical Research, 4Centre for Medical Parasitology, Department of Infectious Diseases,Rigshospitalet
Chapitres
- 00:04Introduction
- 00:33THP-1 and Infected Erythrocyte (IE) Culture Setup
- 01:58Parasite Staining and Opsonization Plate Preparation
- 03:47THP-1 Cell and Phagocytosis Plate Preparation
- 04:35Phagocytosis Assay
- 06:21Flow Cytometry Acquisition and Analysis
- 07:34Results: Representative Evaluation of Parasite-IE Opsonization and Phagocytosis
- 09:21Conclusion
