/

/

使用三维共培养系统NICO-1评估卵巢癌干细胞样细胞的血管遗传特性

Journal JoVE
Biologie

Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.

Journal JoVE Biologie

Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1

Please note that all translations are automatically generated. Click here for the English version.

使用三维共培养系统NICO-1评估卵巢癌干细胞样细胞的血管遗传特性

DOI:

10.3791/61751-v

•

07:59 min

•

December 05, 2020

•

Yuko Miyagawa, Kazunori Nagasaka, Kaoru Yamawaki³, Yutaro Mori³, Tatsuya Ishiguro, Kei Hashimoto, Ryoko Koike, Siho Fukui, Takeru Sugihara, Takayuki Ichinose, Haruko Hiraike, Koichiro Kido, Koji Okamoto, Takayuki Enomoto, Takuya Ayabe

¹Department of Obstetrics and Gynecology,Teikyo University School of Medicine, ²Department of Obstetrics and Gynecology,Niigata University Graduate School of Medical and Dental Sciences, ³Devision of Cancer Differentiation,National Cancer Center Research Institute

Chapitres

00:05Introduction
00:55Ovarian Cancer Stem Cell (OCSC) Isolation and Culture
02:23CSC Passage
03:24HUEhT-1 Endothelial Cell Culture
04:14NICO-1 Coculture Plate Preparation
05:16NICO-1 System HUEhT-1 and CSC Seeding
06:14Results: Representative CSC-Induced Neovascularization and Vascular Formation
07:19Conclusion

Summary

Traduction automatique

卵巢癌干细胞（OCSC）负责癌症的发生、复发、治疗耐药性和转移。OCSC血管生态位被认为可促进OCSC的自我更新，导致化学耐药性。该协议为体外建立可重复的OCSC血管生态位模型提供了基础。

Tags

Keywords: Ovarian Cancer Cancer Stem Cells Angiogenesis 3D Co-culture System NICO-1 Density Gradient Centrifugation OCSC Medium HUEhT-1 Endothelial Cells Tumor Microenvironment Paracentesis Cell Detachment

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

嗨 - C：一个方法研究基因组的三维架构。

流动小鼠减数分裂细胞的流式细胞仪净化

从三维混合细胞球体共培养的乳腺上皮细胞的隔离

细胞形态变化，从三维荧光图像量化的分析工具，

二维和三维活细胞成像的DNA损伤反应蛋白

使用高解析度电脑断层可视化的三维结构和功能的植物血管

成像使用烟草瞬时表达系统MAPK信号通路的空间重组

在细胞间接触缺失的两个蜂窝型的插入共培养系统的开发

无源清清除小鼠卵巢血管构筑的三维重建

Adipo: 三维脂肪组织成像的组织清除方法

Read Article