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A Step-by-Step Guide to Mosquito Electroantennography
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Journal JoVE Biologie
A Step-by-Step Guide to Mosquito Electroantennography

A Step-by-Step Guide to Mosquito Electroantennography

DOI:
10.3791/62042-v

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06:39 min

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March 10, 2021

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1Department of Biochemistry; The Fralin Life Science Institute; The Global Change Center; Department of Entomology and the Center for Emerging Zoonotic and Arthropod-Borne Pathogens,Virginia Polytechnic Institute and State University

Chapitres

  • 00:05Introduction
  • 00:37Odor Storage and Electrode Holder-Capillary Preparation
  • 02:22Electroantennography (EAG) Rig Preparation
  • 02:57Mosquito Head Preparation and Mounting
  • 04:10Recordings and Data Analysis
  • 04:47Results: Olfactory Mediated Behaviors of Mosquito Species from Various Genera
  • 06:01Conclusion

Summary

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The present article details a step-by-step protocol for successful and low noise electroantennograms in several genera of mosquitoes, including both females and males.

Tags

ElectroantennographyMosquitoesPhysiological StatusInsect ModelsOdorant MixturesBorosilicate CapillariesChloridizationSaline SolutionReference ElectrodeRecording ElectrodeAir TableMedical AirHumidifierControl PulseAluminum PlateWet Cleaning Wipe

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