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Immunology and Infection

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JoVE Journal Immunology and Infection
Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat
 

Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat

Article DOI: 10.3791/62059-v • 04:30 min • February 4th, 2021
February 4th, 2021 •
*1,2, *1, 3,4, 1,3, *2,5, *1,6, *1,6
1Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin and German Centre for Cardiovascular Research (DZHK), 2Department of Experimental and Clinical Medicine, University of Florence, 3Berlin Institute of Health, 4Department of Cardiology, German Heart Centre Berlin (DHZB), 5Sod of Interdisciplinary Internal Medicine, Azienda Ospedaliera Universitaria Careggi (AOUC), 6Department of General and Interventional Cardiology, Helios Klinikum Erfurt
* These authors contributed equally

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résumé
Automatic Translation

In this study, we describe the process of T lymphocyte isolation from fresh samples of calcified aortic valves and the analytical steps of T cell-cloning for the characterization of the adaptive leukocyte subsets by using flow cytometry analysis.

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Keywords: T Lymphocytes Aortic Valve Calcification Feeder Cells Density Gradient Centrifugation Cell Culture PBMC Isolation IL-2 T-cell Cloning T-cell Culture U-bottom 96-well Plates
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