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使用荧光标记肉瘤蛋白缩短多能干细胞衍生心肌细胞的肉瘤。
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Médecine
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Journal JoVE Médecine
Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.
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使用荧光标记肉瘤蛋白缩短多能干细胞衍生心肌细胞的肉瘤。

DOI:
10.3791/62129-v

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08:37 min

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March 03, 2021

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, , , , , , ,
1Division of Regenerative Medicine, Center for Molecular Medicine,Jichi Medical University, 2Department of Pediatrics,Jichi Medical University, 3Institute of Global Innovation Research,Tokyo University of Agriculture and Technology, 4Department of Precision Mechanics, Faculty of Science and Engineering,Chuo University

Chapitres

  • 00:04Introduction
  • 01:04Human Pluripotent Stem Cell Differentiation
  • 03:15Patterned Pluripotent Stem Cell-Derived Cardiomyocyte (PSC-CM) Culture
  • 05:07Time-Lapse Sarcomere Imaging
  • 05:58Time-Lapse Imaging Analysis
  • 06:36Results: Representative Sarcomere Shortening Evaluation
  • 07:56Conclusion

Summary

Traduction automatique

Traduction automatique

该方法可用于使用多能干细胞衍生心肌细胞与荧光标记肉瘤蛋白来检查肉瘤缩短。

Tags

Sarcomere ShorteningPluripotent Stem Cell-derived CardiomyocytesFluorescent-tagged Sarcomere ProteinsAAV-based TransductionInduced Pluripotent Stem CellsCardiomyopathy TherapySkeletal Muscle DysfunctionMyopathyLaminin-511 E8ROCK InhibitorGSK-3 InhibitorPorcupine Inhibitor

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