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Creazione di un sistema di coltura sferoide epidermica ad alto rendimento per modellare la plasticità delle cellule staminali dei cheratinociti
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1Department of Pathology, Microbiology and Immunology,University of South Carolina School of Medicine, 2Department of Dermatology,Brigham and Women’s Hospital, Harvard Medical School, 3Department of Biochemistry and Molecular Biology,University of Maryland School of Medicine Baltimore, 4Department of Pathology,Albert Einstein College of Medicine, 5Department of Pediatrics,Vanderbilt University Medical Center, 6Department of Drug Discovery and Biomedical Sciences, College of Pharmacy,University of South Carolina
Chapitres
- 00:04Introduction
- 00:27Isolation and Culture of Human Keratinocytes from Neonatal Foreskin Tissue
- 02:37Generating Skin Epidermosphere Cultures in Vitro
- 05:02Epidermal Spheroid Re-Plating Assay
- 05:42Characterization of Spheroid-Derived (SD) Sub-Populations by FACS
- 08:06Results: Characterization of Epidermal Spheroid Formation and Spheroid-Derived NHKs
- 09:30Conclusion
Qui descriviamo un protocollo per la coltivazione sistematica di sferoidi epidermici in coltura di sospensione 3D. Questo protocollo ha applicazioni ad ampio raggio per l'uso in una varietà di tipi di tessuto epiteliale e per la modellazione di diverse malattie e condizioni umane.
