Journal
/
Immunologie et infection
/
Dobbel merking av immunfluorescens ved hjelp av antistoffer fra samme art for å studere vertspatogeninteraksjoner
Journal JoVE
Immunologie et infection
Author Produced
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions

Dobbel merking av immunfluorescens ved hjelp av antistoffer fra samme art for å studere vertspatogeninteraksjoner

DOI:
10.3791/62219-v

•

07:35 min

•

July 10, 2021

•

, ,
1Laboratory of Cell and Molecular Biology of Trypanosomatids, Department of Cellular and Molecular Biology and Pathogenic Bioagents, Ribeirao Preto Medical School,University of Sao Paulo, Ribeirao Preto, SP, Brazil

Chapitres

  • 00:04Introduction
  • 01:21Parasite Cultures
  • 02:00Control Immunofluorescence Protocol
  • 03:23Double Labeling Immunofluorescence ProtocolUsing Monoclonal and Polyclonal Antibodies Raised in the Same Host
  • 04:18Triple Labeling Immunofluorescence Protocol with the Addition of the Third Polyclonal Antibody from Different Host Species
  • 05:16Confocal Imaging Acquisition
  • 05:39Results: Host-pathogen Interactions Studies Using Double Labeling Immunofluorescence
  • 06:51Conclusion

Summary

Traduction automatique

Traduction automatique

Her beskriver protokollen hvordan man utfører dobbeltmerking av immunfluorescens ved hjelp av primære antistoffer oppdratt i samme art for å studere vertspatogeninteraksjoner. Det kan også inkludere det tredje antistoffet fra en annen vert i denne protokollen. Denne tilnærmingen kan gjøres i hvilken som helst celletype og patogener.

Tags

Double LabelingImmunofluorescenceAntibodiesSame SpeciesHost-pathogen InteractionsTrypanosoma CruziLLC-MK2 CellsPolyclonal AntibodyMonoclonal AntibodyPhalloidinActin Filaments

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/62219?article_type=v&slug=double-labeling-immunofluorescence-using-antibodies-from-same-species

Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging
check_url/fr/62219?article_type=v&slug=double-labeling-immunofluorescence-using-antibodies-from-same-species

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
check_url/fr/62219?article_type=v&slug=double-labeling-immunofluorescence-using-antibodies-from-same-species

Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions
check_url/fr/62219?article_type=v&slug=double-labeling-immunofluorescence-using-antibodies-from-same-species

En komparativ tilnærming til preger landskapet av Host-patogenfrie Protein-protein interaksjoner
check_url/fr/62219?article_type=v&slug=double-labeling-immunofluorescence-using-antibodies-from-same-species

Imaging inlc Secretion å etterforske Cellular Infeksjon av Bakteriell Patogen<em> Listeria monocytogenes</em
check_url/fr/62219?article_type=v&slug=double-labeling-immunofluorescence-using-antibodies-from-same-species

Isolering av Double Negative αβ T celler fra nyre
check_url/fr/62219?article_type=v&slug=double-labeling-immunofluorescence-using-antibodies-from-same-species

Ved å bruke<em> Shigella flexneri</em> Å studere autofagi-Cytoskjelettet Interactions
check_url/fr/62219?article_type=v&slug=double-labeling-immunofluorescence-using-antibodies-from-same-species

Generasjon av Escape varianter av nøytralisere influensa Virus monoklonale antistoffer
check_url/fr/62219?article_type=v&slug=double-labeling-immunofluorescence-using-antibodies-from-same-species

Evaluering av Host-patogen svar og vaksinen i mus
check_url/fr/62219?article_type=v&slug=double-labeling-immunofluorescence-using-antibodies-from-same-species

Menneskelig Colonoid Monolayers å studere interaksjonen mellom patogener, Commensals og vert Intestinal epitel

Read Article