Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Department of Physiology & The Institute for Digital Medicine (WisDM),National University of Singapore, 2College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering, 3Mechanobiology Institute,National University of Singapore, 4Institute of Bioengineering and Nanotechnology,A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering, 6CAMP,Singapore-MIT Alliance for Research and Technology
Chapitres
- 00:04Introduction
- 00:45Portal Vein Cannulation
- 03:54Liver Perfusion, Digestion, Hepatocyte Isolation
- 05:30Results: Estimation of Purity and Function of Isolated Hepatocytes
- 07:07Conclusion
This protocol details the use of a special intravenous catheter, standardized sterile disposable tubing, temperature control complemented by real-time monitoring, and an alarm system for two-step collagenase perfusion procedure to improve the consistency in the viability, yield, and functionality of isolated primary rat hepatocytes.
