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使用膜结合IL-21修饰B细胞系的自然杀伤(NK)和CAR-NK细胞扩增方法
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line
DOI:

11:02 min

February 08, 2022

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Chapitres

  • 00:04introduction
  • 00:54Natural Killer (NK) Cell Expansion from Liver Tissues: Day 0
  • 03:14Primary Natural Killer (NK) Cell Expansion from Peripheral Blood Mononuclear Cells (PBMCs): Day 0
  • 04:34Attachment of 293T Cells (Day 2) and Retrovirus Transfection (Day 3)
  • 05:58Retronectin Plate-Coating (Day 3) and Transduction (Day 4)
  • 08:18CAR-NK Cells Collection (Day 6 or 7)
  • 09:04Results: Expansion of PBNK Cells and Flow Cytometric Analysis of CAR-NK Cells
  • 10:22Conclusion

Summary

Traduction automatique

在这里,我们提出了一种扩增外周血自然杀伤(PBNK),来自肝组织的NK细胞以及源自外周血单核细胞(PBMC)或脐带血(CB)的嵌合抗原受体(CAR)-NK细胞的方法。该协议展示了使用221-mIL-21饲养层细胞的NK和CAR-NK细胞的扩增,以及扩增的NK细胞的优化纯度。

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