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Chapitres
- 00:04Introduction
- 00:47Optical Setup
- 01:22Silica Beads Preparation
- 03:24Trap Position, Power, and Stiffness Calibration
- 04:53Sample Assembly
- 05:54Sample Measurement and Actin‐Myosin Contact Establishment
- 07:37Results: Position Recording and Force Inversion
- 08:53Conclusion
Presented here is a comprehensive protocol to perform ultrafast force-clamp experiments on processive myosin-5 motors, which could be easily extended to the study of other classes of processive motors. The protocol details all the necessary steps, from the setup of the experimental apparatus to sample preparation, data acquisition and analysis.
Tags
Keywords: Mechanoenzymatic PropertiesProcessive MyosinsUltrafast Force-clamp SpectroscopyLaser TweezersChemomechanicsMyosin MotorsForce ProductionForce DirectionalityProcessive MotorsKinesinsDyneinsForce RegulationAcousto-optic DeflectorsIrisObjectiveLaser BeamSilica BeadsPentyl AcetateAcetoneNitrocelluloseEthanolGlass CoverslipNitrogen FlowDouble-sided Tape
