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Microinjection of Corn Planthopper, Peregrinus maidis, Embryos for CRISPR/Cas9 Genome Editing
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Microinjection of Corn Planthopper, Peregrinus maidis, Embryos for CRISPR/Cas9 Genome Editing

Microinjection of Corn Planthopper, Peregrinus maidis, Embryos for CRISPR/Cas9 Genome Editing

DOI:
10.3791/62417-v

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07:27 min

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March 26, 2021

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, , , , , ,
1Department of Entomology and Plant Pathology,North Carolina State University

Chapitres

  • 00:04Introduction
  • 01:08Preparation of Agarose‐Based Egg‐Lay Chamber
  • 02:23Embryo Collection and Alignment
  • 02:59Microinjection and Post‐Injection Care
  • 03:48Incubation and Hatching of Embryos
  • 05:03Results: Microinjection of P. maidis Embryos for CRISPR/Cas9 Genome Editing and Collection
  • 06:32Conclusion

Summary

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Herein are protocols for collecting and microinjecting precellular corn planthopper embryos for the purpose of modifying their genome via CRISPR/Cas9-based genome editing or for the addition of marked transposable elements through germline transformation.

Tags

MicroinjectionCorn PlanthopperPeregrinus MaidisCRISPR/Cas9Genome EditingHemipteransTransgenicInsect EmbryosDesiccationThripsGene FunctionPest Control

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