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Análisis del microambiente hepático durante la progresión temprana del carcinoma hepatocelular asociado a la enfermedad del hígado graso no alcohólico en pez cebra
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1Department of Developmental and Molecular Biology,Albert Einstein College of Medicine, 2Department of Medicine (Hepatology),Albert Einstein College of Medicine, 3Einstein-Mount Sinai Diabetes Research Center,Albert Einstein College of Medicine, 4Marion Bessin Liver Research Center,Albert Einstein College of Medicine
Chapitres
- 00:05Introduction
- 01:08Preparation of 10% Cholesterol-Enriched Diet for Acute Cholesterol Surplus
- 02:29NASH Induction with Short-Term Larvae Feeding with Cholesterol-Enriched Diet Static Conditions
- 04:22Collection of 13 Days Post-Fertilization Larvae from Feeding Boxes
- 05:06Non-Invasive Confocal Imaging Using the Zebrafish Wounding and Entrapment Device for Growth and Imaging (zWEDGI)
- 05:54Immune Cell Recruitment Analysis
- 06:49Results: Monitoring Liver Disease Progression in the Hepatocellular Carcinoma (HCC) Zebrafish Model
- 08:30Conclusion
Aquí, presentamos cómo generar un modelo de pez cebra de carcinoma hepatocelular (HCC) asociado a la enfermedad del hígado graso no alcohólico (NAFLD) para estudiar el impacto del excedente de colesterol en el microambiente hepático y el paisaje de las células inmunes.
