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Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter
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Immunologie et infection
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Journal JoVE Immunologie et infection
Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

DOI:
10.3791/62488-v

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07:44 min

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August 12, 2021

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, , , , , , ,
1Ottawa Hospital Research Institute, 2Department of Biochemistry, Microbiology and Immunology,University of Ottawa, 3Department of Biotechnology,University of Tehran

Chapitres

  • 00:05Introduction
  • 00:50Production and Evaluation of the HiBiT-RBD Bioreporter
  • 03:47Detecting Anti-RBD Antibody with a Fast and Sensitive Assay
  • 04:53High-Throughput Detection of the SARS-CoV-2-Specific Antibodies from Patient Serum Samples
  • 06:05Results: SARS-CoV-2 Antibody Detection Using HiBiT-Based Bioreporter
  • 07:06Conclusion

Summary

Traduction automatique

Traduction automatique

The outlined protocol describes the procedure for producing the HiBiT-receptor-binding domain protein complex and its application for fast and sensitive detection of SARS-CoV-2 antibodies.

Tags

SARS-CoV-2Receptor-binding DomainAntibody DetectionHiBiT-based BioreporterCOVID-19 ResearchHigh-throughput AssayCell CultureTransfectionPassive Lysis Buffer

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