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Bioengineering

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JoVE Journal Bioengineering
Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture
 

Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture

Article DOI: 10.3791/62519-v • 09:32 min • May 11th, 2021
May 11th, 2021 •
1, 1, 1, 1,2
1Nanotechnology Research Center, National Research Council Canada, 2Department of Medical Microbiology and Immunology, University of Alberta

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This protocol highlights a method to rapidly assess the biocompatibility of a crystalline nanocellulose (CNC)/agarose composite hydrogel biomaterial ink with mouse bone marrow-derived mast cells in terms of cell viability and phenotypic expression of the cell surface receptors, Kit (CD117) and high-affinity IgE receptor (FcεRI).

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Keywords: Nanocellulose Agarose Biomaterial Ink Bone Marrow-derived Mast Cells 3D Bioprinting Cytotoxicity Tissue Engineering Biocompatible Bioprinter Bioprinting Nozzle Print Bed Calibration
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