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Bead Loading Proteins and Nucleic Acids into Adherent Human Cells
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Biologie
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Journal JoVE Biologie
Bead Loading Proteins and Nucleic Acids into Adherent Human Cells

Bead Loading Proteins and Nucleic Acids into Adherent Human Cells

DOI:
10.3791/62559-v

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07:28 min

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June 01, 2021

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, , , ,
1Department of Biochemistry and Molecular Biology,Colorado State University, 2World Research Hub Initiative, Institute of Innovative Research,Tokyo Institute of Technology

Chapitres

  • 00:04Introduction
  • 01:00Clean, Sterilize, and Dry Glass Beads
  • 02:26Assemble the Bead Loader Apparatus
  • 03:18Bead Loading Cells and Imaging the Bead-Loaded Cells
  • 04:43Results: Proteins and Nucleic Acids Bead-loaded onto Adherent Human Cells
  • 06:49Conclusion

Summary

Traduction automatique

Traduction automatique

Bead loading introduces proteins, plasmids, and particles into adherent mammalian cells. This cell loading technique is inexpensive, rapid, and does not substantially affect cell health. It is best suited for live-cell imaging.

Tags

Bead LoadingAdherent CellsSingle Cell MicroscopyProteinsNucleic AcidsCell LoadingSodium HydroxideEthanolBiosafetyPolypropylene MeshUV SterilizationDesiccant

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