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Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
Journal JoVE
Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

DOI:
10.3791/62663-v

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13:53 min

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August 26, 2021

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, ,
1Department of Chemical Pathology, Faculty of Health Sciences,University of Pretoria, 2Tshwane Academic Division,National Health Laboratory Service

Chapitres

  • 00:04Introduction
  • 00:43Preparation of Cell Culture and Treatments for Flow Cytometry
  • 02:56Enumerating Cells Using the Viability Assay
  • 03:36Staining Cells for Flow Cytometry
  • 08:00Run Samples on the Flow Cytometer
  • 10:04Post-Acquisition and Statistical Analysis
  • 11:20Results: Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
  • 13:01Conclusion

Summary

Traduction automatique

Traduction automatique

Apoptosis can be characterized by flow cytometric analysis of early and late apoptotic biomarkers. The cervical cancer cell line, SiHa, was analyzed for apoptosis biomarkers after treatment with Actinomycin D using a benchtop flow cytometer.

Tags

Keywords: Flow CytometryApoptosisActinomycin DSiHa Cervical Cancer CellsBiomarkersAnnexin VCell CultureTrypsinPhosphatidylserineDNA Binding Dyes

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