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Aktinomisin D ile Tedavi Edilen SiHa Serviks Kanseri Hücrelerinde Apoptotik Biyobelirteçlerin Akış Sitometrik Analizi
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
DOI:

13:53 min

August 26, 2021

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Chapitres

  • 00:04Introduction
  • 00:43Preparation of Cell Culture and Treatments for Flow Cytometry
  • 02:56Enumerating Cells Using the Viability Assay
  • 03:36Staining Cells for Flow Cytometry
  • 08:00Run Samples on the Flow Cytometer
  • 10:04Post-Acquisition and Statistical Analysis
  • 11:20Results: Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
  • 13:01Conclusion

Summary

Traduction automatique

Apoptoz, erken ve geç apoptotik biyobelirteçlerin akış sitometrik analizi ile karakterize edilebilir. Serviks kanseri hücre hattı SiHa, bir tezgah üstü akış sitometresi kullanılarak Actinomycin D ile tedaviden sonra apoptoz biyobelirteçleri için analiz edildi.

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