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选择性清洁野生 卡诺菌线 虫以富集肠道微生物组细菌
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria
DOI:

09:47 min

August 13, 2021

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Chapitres

  • 00:04Introduction
  • 00:34Inducing Dauer Formation for Wild Nematodes on Nematode Growth Medium (NGM) Plates
  • 01:16Washing and Disinfecting the Nematodes
  • 03:12Removing the Antibiotic and SDS Solution
  • 04:08Propagate a Clean Nematode Strain
  • 05:27Intestinal Dissection and PCR Identification of Microbial Species
  • 08:17Results: The Host-microbe Interactions in the Context of the Intestines of Caenorhabditis tropicalis Strain JU1848
  • 09:16Conclusion

Summary

Traduction automatique

野生 卡诺菌线虫 与许多微生物有关,通常在肠腔内或感染肠道。该协议详细介绍了一种利用dauer角质层的抗性来丰富无法培养的微生物定植于肠道的方法。

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