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Dissection and Immunostaining of Larval Salivary Glands from Anopheles gambiae Mosquitoes
 

Dissection and Immunostaining of Larval Salivary Glands from Anopheles gambiae Mosquitoes

Article DOI: 10.3791/62989-v • 06:12 min • September 30th, 2021
September 30th, 2021 •
1,2, 1, 1, 3, 1
1Department of Cell Biology, The Johns Hopkins University School of Medicine, 2Tufts School of Medicine, 3Department of Biomedical Sciences, Idaho College of Osteopathic Medicine

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The adult mosquito salivary gland (SG) is required for the transmission of all mosquito-borne pathogens to their human hosts, including viruses and parasites. This video demonstrates efficient isolation of the SGs from the larval (L4) stage Anopheles gambiae mosquitoes and preparation of the L4 SGs for further analysis.

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Keywords: Salivary Glands Anopheles Gambiae Mosquito Larvae Dissection Immunostaining Disease Transmission Malarial Parasites Rab 11 Alexa Fluor 488 Nile Red Hoechst
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