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Chapitres
- 00:00Titre
- 00:40Introduction
- 02:19Preparing the PCR Product for Pyrosequencing
- 06:42Pyrosequencing
- 06:45Entering Assay Details Using Pyrosequencing 96A1.1 Software
- 08:36Run Sample Plate
- 11:16Examining Pyrosequencing Results
- 12:18Conclusion
Pyrosequencing(R) is one of the most thorough yet simple methods to date used to analyze polymorphisms. This method has led to rapid and efficient single-nucleotide polymorphism evaluation including many clinically relevant polymorphisms. The technique and methodology of Pyrosequencing is explained.
Tags
PyrosequencingGenotypingPharmacogenetic ResearchHuman Genome ProjectDataGenotyping MethodsPolymorphismsTri-allelicIndelsShort-repeat PolymorphismsAllele PercentagesMethylationPooled Sample AssessmentInternal Quality ControlSingle-nucleotide Polymorphism EvaluationClinically Relevant PolymorphismsTechniqueMethodology
