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Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation
Journal JoVE
Bio-ingénierie
This content is Free Access.
Journal JoVE Bio-ingénierie
Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation
DOI:

07:28 min

November 04, 2021

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Chapitres

  • 00:04Introduction
  • 00:42Hydrogel Formation on Glass Coverslip
  • 01:57Hydrogel Formation Over Microwell Arrays
  • 03:00Hydrogel Degradation with the Patterned Illumination Tool
  • 04:13Cell Retrieval
  • 04:55Results: Cell Viability Assessment
  • 06:58Conclusion

Summary

Traduction automatique

The use of photodegradable hydrogels to isolate bacterial cells by utilizing a high-resolution light pattering tool is reported. Essential experimental procedures, results, and advantages of the process are reviewed. The method enables rapid and inexpensive isolation of targeted bacteria showing rare or unique functions from heterogeneous communities or populations.

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