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JoVE Journal
Bioengineering

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Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation
 

Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation

Article DOI: 10.3791/63048-v 07:28 min November 4th, 2021
November 4th, 2021

Chapters

Summary

The use of photodegradable hydrogels to isolate bacterial cells by utilizing a high-resolution light pattering tool is reported. Essential experimental procedures, results, and advantages of the process are reviewed. The method enables rapid and inexpensive isolation of targeted bacteria showing rare or unique functions from heterogeneous communities or populations.

Tags

Keywords: Photodegradable Hydrogel Bacterial Screening Cell Isolation Microfluidics Cell Encapsulation Hydrogel Precursor PEG Diacrylate PEG Thiol Microwell Array ATGN Media Cell Suspension Phosphate Buffered Saline
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