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Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues
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Chapters
- 00:05Title
- 01:41Activating the Surface of Glass Coverslips
- 03:42Preparation of Hydroxy-PAAm Hydrogels
- 05:50Micropatterning Hydroxy-PAAm Hydrogels
- 09:09Results: Micropatterned Hydroxy-PAAm Hydrogels Enable Independent Tuning of Mechanotransduction Cues In Vitro
- 10:55Conclusion
We present a new polyacrylamide hydrogel, called hydroxy-PAAm, that allows a direct binding of ECM proteins with minimal cost or expertise. The combination of hydroxy-PAAm hydrogels with microcontact printing facilitates independent control of many cues of the natural cell microenvironment for studying cellular mechanostransduction.
