Journal
/
/
使用水凝胶图案化技术控制细胞粘附,用于牵引力显微镜的应用
Journal JoVE
Bio-ingénierie
Author Produced
This content is Free Access.
Journal JoVE Bio-ingénierie
Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
DOI:

12:26 min

January 29, 2022

, , , , , , ,

Chapitres

  • 00:04Introduction
  • 01:22Preparation of Methacrylated Coverslips
  • 02:39Micropatterning of Extracellular Matrix Proteins on Glass Coverslips
  • 05:31Fabrication of Patterned Polyacrylamide Hydrogels
  • 09:15Local Release of Cell Traction Forces by UV-A Laser Illumination
  • 10:26Image Processing, Particle Image Velocity and Calculation of Traction Forces
  • 10:55Résultats
  • 11:56Conclusion

Summary

Traduction automatique

结合近紫外光刻和牵引力显微镜来测量微图案水凝胶上的细胞力。单细胞的靶向光诱导释放可以对同一样品进行大量测量。

Vidéos Connexes

Read Article