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2光子顕微鏡を使用して、糸球体プロセスに対する慢性片側尿管閉塞の影響を定量化
Journal JoVE
Médecine
This content is Free Access.
Journal JoVE Médecine
Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes
DOI:

11:47 min

March 04, 2022

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Chapitres

  • 00:05Introduction
  • 00:55Preparing the Munich Wistar Frmter or SD Rat for Unilateral Ureteral Obstruction (UUO) Surgery
  • 01:53Surgical Preparation for Intravital 2-Photon Imaging
  • 04:04Positioning the Rat for Imaging and Image Acquisition for Quantitative Analysis
  • 06:16Calculating Red Blood Cell Flow in Surface Glomerular Capillary Loops and Renal Vasculature Using a Linescan Function
  • 07:45Calculating White Blood Cell (WBC) Occlusion in Glomerular Capillary Loops
  • 08:38Results: Quantification of Glomerular Processes in the Unilateral Ureteral Obstruction (UUO) Model of Rats
  • 10:56Conclusion

Summary

Traduction automatique

ここでは、表面糸球体を有するミュンヘンWistar Fromterラットにおける2光子顕微鏡を使用して、糸球体の動態と機能に対する長期の尿管閉塞の影響を定量化するプロトコルを紹介します。

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