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JoVE Journal Neuroscience
Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson's Disease Model
 

Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson's Disease Model

Article DOI: 10.3791/63355-v • 07:32 min • December 28th, 2021
December 28th, 2021 •
1, 1, 1,2, 1, 1, 2, 1
1Collaborative Drug Discovery Research (CDDR) Group and Brain Degeneration and Therapeutics Group, Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Cawangan Selangor, Kampus Puncak Alam, 2Department of Medicine, Faculty of Medicine, Universiti Malaya

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The present protocol describes the intracerebroventricular (ICV) injection of adult zebrafish with neurotoxic 6-hydroxydopamine (6-OHDA) at the ventral diencephalon (Dn) and the assessment of the impairment and subsequent recovery of swimming behavior postlesion by using the open tank test, which is accompanied by analysis using a video tracking software.

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Locomotor Assessment 6-Hydroxydopamine-induced Parkinson's Disease Model Zebrafish Motor Impairment Neurotoxin Injection Dopaminergic Neurons Ablation Functional Effects Setup Neuroregeneration Mechanisms Cell Replacement Treatment Strategies Fish Maintenance Water Tank Sea Salt Temperature Control Light-dark Photoperiod Feeding Regimen Tricaine Methanesulfonate Stock Solution Anesthesia Solution 6-hydroxydopamine Preparation
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